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Increased hydrogen production by Escherichia coli strain HD701 in comparison with the wild-type parent strain MC4100

Penfold, DW and Forster, CF and Macaskie, LE (2003) Increased hydrogen production by Escherichia coli strain HD701 in comparison with the wild-type parent strain MC4100. Enzyme and Microbial Technology, 33 (2-3). pp. 185-189.

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URL of Published Version: http://www.sciencedirect.com/science/article/pii/S0141022903001157

Identification Number/DOI: doi:10.1016/S0141-0229(03)00115-7

Hydrogen production by Escherichia coli is mediated by the formate hydrogenlyase (FHL) complex. E. coli strain HD701 cannot synthesize the FHL complex repressor, Hyc A. Consequently, it has an up-regulated FHL system and can, therefore, evolve hydrogen at a greater rate than its parental wild type, E. coli MC4100. Resting cells of E. coli strain HD701 and MC4100 were set up in batch mode in
phosphate buffered saline (PBS) to decouple growth from hydrogen production at the expense of sugar solutions of varying composition. Strain HD701 evolved several times more hydrogen than MC4100 at glucose concentrations ranging from 3 to 200 mM. The difference in the amount of H2 evolved by both strains decreased as the concentration of glucose increased. The highest rate of H2 evolution by strain HD701was 31ml h−1 ODunit −1 l−1 at a glucose concentration of 100 mM.With strain MC4100, the highest ratewas 16ml h−1 ODunit −1 l−1 under these conditions. Experiments using industrial wastes with a high sugar content yielded similar results. In each case, strain HD701
evolved hydrogen at a faster rate than the wild type, showing a possible potential for commercial hydrogen production.

Type of Work:Article
Date:2003 (Publication)
School/Faculty:Colleges (2008 onwards) > College of Life & Environmental Sciences
Department:School of Biosciences
Subjects:Q Science (General)
Institution:University of Birmingham
Copyright Holders:Elsevier
ID Code:1001
Refereed:YES

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  • Increased hydrogen production by Escherichia coli strain HD701 in comparison with the wild-type parent strain MC4100. (deposited 17 Jul 2012 12:46) [Currently Displayed]
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