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Specific electrochemical iodination of horse heart myoglobin at tyrosine 103 as determined by Fourier transform ion cyclotron resonance mass spectrometry

Iniesta, Jesus and Cooper, Helen J. and Marshall, Alan G. and Heptinstall, John and Walton, David J. and Peterson, Ian R. (2008) Specific electrochemical iodination of horse heart myoglobin at tyrosine 103 as determined by Fourier transform ion cyclotron resonance mass spectrometry. Archives of Biochemistry and Biophysics, 474 (1). pp. 1-7. ISSN 0003-9861

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URL of Published Version: http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=18348862

Identification Number/DOI: 10.1016/j.abb.2008.02.032.

The iodination of proteins remains a useful tool in biochemistry for radiolabelling. However,chemical or enzymatic iodination is difficult to control and can give deleterious polyiodination. Previously, we have shown that electrooxidation with nitrite is a rapid method for the selective nitration of tyrosine residues in proteins. In principle, it should be possible to substitute a number of electrooxidisable anions into the tyrosine phenol ring. Electrochemical iodination is more difficult to control than nitration because the rapid anodic oxidation of I− leads to persistent formation of the iodinating triiodide anion. However, application of pulsed electrooxidation and reduction cycles is shown to be an effective procedure for the selective mono and double-iodination of myoglobin, which may have general application to other proteins in
controlling of the level of iodination. Mono- and double-iodination of myoglobin by this method was confirmed by electrospray FT-ICR mass spectrometry. Infrared multiphoton dissociation (IRMPD) enabled localization of the site of mono-iodination to be restricted to either His97 or Tyr103. More extensive sequence coverage was obtained with electron capture dissociation (ECD), allowing unambiguous assignment of the site of iodination to Tyr103.

Type of Work:Article
Date:01 June 2008 (Publication)
School/Faculty:Schools (1998 to 2008) > School of Biosciences
Department:Structural Biology
Subjects:Q Science (General)
Institution:University of Birmingham
Copyright Holders:Elsevier
ID Code:116
Refereed:YES
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