ePrints Repository

Open-source genomic analysis of Shiga-toxin–producing E. coli O104:H4

Rohde, Holger and Qin, Junjie and Cui, Yujun and Li, Dongfang and Loman, N. J. and Hentschke, Moritz and Chen, Wentong and Pu, Fei and Peng, Yangqing and Li, Junhua and Xi, Feng and Li, Shenghui and Li, Yin and Zhang, Zhaoxi and Yang, Xianwei and Zhao, Meiru and Wang, Peng and Guan, Yuanlin and Cen, Zhong and Zhao, Xiangna and Christner, Martin and Kobbe, Robin and Loos, Sebastian and Oh, Jun and Yang, Liang and Danchin, Antoine and Gao, George F. and Song, Yajun and Li, Yingrui and Yang, Huanming and Wang, Jian and Xu, Jianguo and Pallen, Mark J. and Wang, Jun and Aepfelbacher, Martin and Yang, Ruifu (2011) Open-source genomic analysis of Shiga-toxin–producing E. coli O104:H4. New England Journal of Medicine, 365 (8). pp. 718-724. ISSN 0028-4793

Loading
PDF (743Kb)

URL of Published Version: http://dx.doi.org/10.1056/nejmoa1107643

Identification Number/DOI: 10.1056/nejmoa1107643

An outbreak caused by Shiga-toxin–producing Escherichia coli O104:H4 occurred in Germany in May and June of 2011, with more than 3000 persons infected. Here, we report a cluster of cases associated with a single family and describe an open-source genomic analysis of an isolate from one member of the family. This analysis involved the use of rapid, bench-top DNA sequencing technology, open-source data release, and prompt crowd-sourced analyses. In less than a week, these studies revealed that the outbreak strain belonged to an enteroaggregative E. coli lineage that had acquired genes for Shiga toxin 2 and for antibiotic resistance.

Type of Work:Article
Date:2011 (Publication)
School/Faculty:Colleges (2008 onwards) > College of Life & Environmental Sciences
Department:School of Biosciences
Subjects:QH426 Genetics
QR180 Immunology
RA Public aspects of medicine
Institution:University of Birmingham
Copyright Holders:Massachusetts Medical Society, the authors
ID Code:1165
Refereed:YES
Local Holdings:
Export Reference As : ASCII + BibTeX + Dublin Core + EndNote + HTML + METS + MODS + OpenURL Object + Reference Manager + Refer + RefWorks
Share this item :
QR Code for this page

Repository Staff Only: item control page