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The PRH/Hex repressor protein causes nuclear retention of Groucho/TLE corepressors.

Desjobert, Cecile and Noy, Peter and Swingler, Tracey and Williams, Hannah and Gaston, Kevin and Jayaraman, Padma-Sheela (2008) The PRH/Hex repressor protein causes nuclear retention of Groucho/TLE corepressors. Biochemical Journal. ISSN 0264-6021

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URL of Published Version: http://www.biochemj.org/bj/imps/abs/BJ20080872.htm#

Identification Number/DOI: doi:10.1042/BJ20080872

The Proline-Rich Homeodomain protein (PRH/Hex) is a transcription factor that functions as an important regulator of vertebrate development and many other processes in the adult including haematopoiesis. The Groucho/TLE family of co-repressor proteins also regulate development and modulate the activity of many DNA-binding transcription factors during a range of diverse cellular processes including haematopoiesis. We have shown previously that PRH is a repressor of transcription in haematopoietic cells and that an Eh-1 motif present within the N-terminal transcription repression domain of PRH mediates binding to Groucho/TLE proteins and enables co-repression. Here we demonstrate that PRH regulates the nuclear retention of TLE proteins during cellular fractionation. We show that transcriptional repression and the nuclear retention of TLE proteins requires PRH to bind to both TLE and DNA. In addition, we characterise a trans-dominant negative PRH protein that inhibits wild type PRH activity by sequestering TLE proteins to specific sub-nuclear domains. These results demonstrate that transcriptional repression by PRH is dependent on TLE availability and suggest that sub-nuclear localisation of TLE plays an important role in transcriptional repression by PRH.

Type of Work:Article
Date:19 August 2008 (Publication)
School/Faculty:Schools (1998 to 2008) > School of Medicine
Department:Division of Immunity and Infection
Subjects:R Medicine (General)
Institution:University of Birmingham
Copyright Holders:Portland Press
ID Code:126
Refereed:YES
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