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Bacterial Genome Partitioning: N-Terminal Domain of IncC Protein Encoded by Broad-Host-Range Plasmid RK2 Modulates Oligomerisation and DNA Binding

Batt, S.M and Bingle, Lewis E. H. and Dafforn, T.R and Thomas, Christopher M. (2009) Bacterial Genome Partitioning: N-Terminal Domain of IncC Protein Encoded by Broad-Host-Range Plasmid RK2 Modulates Oligomerisation and DNA Binding. Journal of Molecular Biology, 385 (5). pp. 1361-1374. ISSN 0022-2836

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URL of Published Version: http://www.sciencedirect.com/science/journal/00222836

Identification Number/DOI: 10.1016/j.jmb.2008.12.016

ParAWalker ATPases form part of the machinery that promotes better-thanrandom segregation of bacterial genomes. ParA proteins normally occur in one of two forms, differing by their N-terminal domain (NTD) of approximately 100 aa, which is generally associated with site-specific DNA binding. Unusually, and for as yet unknown reasons, parA (incC) of IncP-1 plasmids is translated from alternative start codons producing two
forms, IncC1 (364 aa) and IncC2 (259 aa), whose ratio varies between hosts.IncC2 could be detected as an oligomeric form containing dimers, tetramers
and octamers, but the N-terminal extension present in IncC1 favours nucleotide-stimulated dimerisation as well as high-affinity and ATPdependent non-specific DNA binding. The IncC1 NTD does not dimerise or bind DNA alone, but it does bind IncC2 in the presence of nucleotides. Mixing IncC1 and IncC2 improved polymerisation and DNA binding. Thus,the NTD may modulate the polymerisation interface, facilitating polymerisation/
depolymerisation and DNA binding, to promote the cycle that
drives partitioning.

Type of Work:Article
Date:06 February 2009 (Publication)
School/Faculty:Schools (1998 to 2008) > School of Biosciences
Department:Biosciences
Subjects:R Medicine (General)
QH301 Biology
QH426 Genetics
Institution:University of Birmingham
Copyright Holders:Elsevier
ID Code:163
Refereed:YES
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