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Large scale localization of protein phosphorylation by use of electron capture dissociation mass spectrometry.

Sweet, Steve M. M. and Bailey, Christopher M and Cunningham, Debbie L. and Heath, John K and Cooper, Helen J. (2009) Large scale localization of protein phosphorylation by use of electron capture dissociation mass spectrometry. Molecular & cellular proteomics : MCP, 8 (5). pp. 904-12. ISSN 1535-9484

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URL of Published Version: http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=2689766&tool=pmcentrez

Identification Number/DOI: 10.1074/mcp.M800451-MCP200

We used on-line electron capture dissociation (ECD) for the large scale identification and localization of sites of phosphorylation. Each FT-ICR ECD event was paired with a linear ion trap collision-induced dissociation (CID) event, allowing a direct comparison of the relative merits of ECD and CID for phosphopeptide identification and site localization. Linear ion trap CID was shown to be most efficient for phosphopeptide identification, whereas FT-ICR ECD was superior for localization of sites of phosphorylation. The combination of confident CID and ECD identification and confident CID and ECD localization is particularly valuable in cases where a phosphopeptide is identified just once within a phosphoproteomics experiment.

Type of Work:Article
Date:01 January 2009 (Publication)
Department:Biosciences
Subjects:Q Science (General)
Institution:University of Birmingham
Copyright Holders:American Society for Biochemistry and Molecular Biology
ID Code:226
Refereed:YES
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