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Kinetics and cellular site of glycolipid loading control

Im, Jin S. and Arora, Pooja and Bricard, Gabriel and Molano, Alberto and Venkataswamy, Manjunatha M. and Baine, Ian and Jerud, Elliot S. and Goldberg, Michael F. and Baena, Andres and Yu, Karl O.A. and Ndonye, Rachel M. (2009) Kinetics and cellular site of glycolipid loading control. Immunity, 30 (6). pp. 888-898. ISSN 1074-7613

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URL of Published Version: http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=19538930

Identification Number/DOI: 10.1016/j.immuni.2009.03.022

CD1d-restricted natural killer T cells (NKT cells) possess a wide range of effector and regulatory
activities that are related to their ability to secrete both T helper 1 (Th1) cell- and Th2 cell-type
cytokines. We analyzed presentation of NKT cell activating α galactosylceramide (αGalCer) analogs
that give predominantly Th2 cell-type cytokine responses to determine how ligand structure controls
the outcome of NKT cell activation. Using a monoclonal antibody specific for αGalCer-CD1d
complexes to visualize and quantitate glycolipid presentation, we found that Th2 cell-type cytokinebiasing
ligands were characterized by rapid and direct loading of cell-surface CD1d proteins.
Complexes formed by association of these Th2 cell-type cytokine-biasing αGalCer analogs with
CD1d showed a distinctive exclusion from ganglioside-enriched, detergent-resistant plasma
membrane microdomains of antigen-presenting cells. These findings help to explain how subtle
alterations in glycolipid ligand structure can control the balance of proinflammatory and antiinflammatory
activities of NKT cells.

Type of Work:Article
Date:19 June 2009 (Publication)
School/Faculty:Colleges (2008 onwards) > College of Life & Environmental Sciences
Department:School of Biosciences
Subjects:QR180 Immunology
Institution:University of Birmingham
Copyright Holders:Elsevier
ID Code:242
Refereed:YES
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