Butala, Matej and Busby, Stephen J. W. and Lee, David J (2009) DNA Sampling: a method for probing protein binding at specific loci on bacterial chromosomes. Nucleic Acids Research, 37 (5). ISSN 0305-1048
| URL of Published Version: http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=19181705 Identification Number/DOI: 10.1093/nar/gkp043 We describe a protocol, DNA sampling, for the rapid isolation of specific segments of DNA, together with bound proteins, from Escherichia coli K-12. The DNA to be sampled is generated as a discrete fragment within cells by the yeast I-SceI meganuclease, and is purified using FLAG-tagged LacI repressor and beads carrying anti-FLAG antibody. We illustrate the method by investigating the proteins bound to the colicin K gene regulatory region, either before or after induction of the colicin K gene promoter. |
| Type of Work: | Article |
|---|---|
| Date: | 01 April 2009 (Publication) |
| School/Faculty: | Colleges (2008 onwards) > College of Life & Environmental Sciences |
| Department: | School of Biosciences |
| Subjects: | Q Science (General) R Medicine (General) |
| Institution: | University of Birmingham |
| Copyright Holders: | Oxford University Press |
| ID Code: | 249 |
| Refereed: | YES |
| Local Holdings: |
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