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Complete Genome Sequence and Comparative Metabolic Profiling of the Prototypical Enteroaggregative Escherichia coli Strain 042

Chaudhuri, Roy R. and Sebaihia, Mohammed and Hobman, Jon L. and Webber, Mark A. and Leyton, Denisse L and Goldberg, Martin D and Cunningham, Adam F. and Scott-Tucker, Anthony and Ferguson, Paul R and Thomas, Christopher M. and Frankel, Gad and Tang, Christoph M. and Dudley, Edward G and Roberts, Ian S and Rasko, David A and Pallen, Mark J. and Parkhill, Julian and Nataro, James P and Thomson, Nicholas R and Henderson, Ian R. (2010) Complete Genome Sequence and Comparative Metabolic Profiling of the Prototypical Enteroaggregative Escherichia coli Strain 042. Plos One, 5 (1). ISSN 1932-6203

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URL of Published Version: http://www.plosone.org/article/info:doi%2F10.1371%2Fjournal.pone.0008801

Identification Number/DOI: 10.1371/journal.pone.0008801

Background
Escherichia coli can experience a multifaceted life, in some cases acting as a commensal while in other cases causing intestinal and/or extraintestinal disease. Several studies suggest enteroaggregative E. coli are the predominant cause of E. coli-mediated diarrhea in the developed world and are second only to Campylobacter sp. as a cause of bacterial-mediated diarrhea. Furthermore, enteroaggregative E. coli are a predominant cause of persistent diarrhea in the developing world where infection has been associated with malnourishment and growth retardation.

Methods
In this study we determined the complete genomic sequence of E. coli 042, the prototypical member of the enteroaggregative E. coli, which has been shown to cause disease in volunteer studies. We performed genomic and phylogenetic comparisons with other E. coli strains revealing previously uncharacterised virulence factors including a variety of secreted proteins and a capsular polysaccharide biosynthetic locus. In addition, by using Biolog™ Phenotype Microarrays we have provided a full metabolic profiling of E. coli 042 and the non-pathogenic lab strain E. coli K-12. We have highlighted the genetic basis for many of the metabolic differences between E. coli 042 and E. coli K-12.

Conclusion
This study provides a genetic context for the vast amount of experimental and epidemiological data published thus far and provides a template for future diagnostic and intervention strategies.

Type of Work:Article
Date:20 January 2010 (Publication)
School/Faculty:Colleges (2008 onwards) > College of Life & Environmental Sciences
Department:School of Immunity and Infection
Subjects:R Medicine (General)
QH426 Genetics
QR Microbiology
Institution:University of Birmingham
Copyright Holders:Public Library of Science
ID Code:300
Refereed:YES
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