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Targets for the MalI repressor at the divergent Escherichia coliK-12malX-malI promoters

Lloyd, Georgina S. and Godfrey, Rita E. and Busby, Stephen J.W. (2010) Targets for the MalI repressor at the divergent Escherichia coliK-12malX-malI promoters. FEMS Microbiology Letters, 305 (1). p. 28. ISSN 03781097

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URL of Published Version: http://dx.doi.org/10.1111/j.1574-6968.2010.01907.x

Identification Number/DOI: doi:10.1111/j.1574-6968.2010.01907.x

Random mutagenesis has been used to identify the target DNA sites for the MalI repressor at the divergent Escherichia coli K-12 malX-malI promoters. The malX promoter is repressed by MalI binding to a DNA site located from position -24 to position -9, upstream of the malX promoter transcript start. The malI promoter is repressed by MalI binding from position +3 to position +18, downstream of the malI transcript start. MalI binding at the malI promoter target is not required for repression of the malX promoter. Similarly, MalI binding at the malX promoter target is not required for repression of the malI. Although the malX and malI promoters are regulated by a single DNA site for cyclic AMP receptor protein, they function independently and each is repressed by MalI binding to a different independent operator site.

Type of Work:Article
Date:2010 (Publication)
School/Faculty:Colleges (2008 onwards) > College of Life & Environmental Sciences
Department:Biosciences
Subjects:QR Microbiology
Institution:University of Birmingham
Copyright Holders:FEMS Microbiology
ID Code:310
Refereed:YES
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