Krone, Nils and Hughes, Beverly and Lavery, Gareth G. and Stewart, P M and Shackleton, Cedric H.L. and Arlt, Wiebke (2010) Gas chromatography/mass spectrometry (GC/MS) remains a pre-eminent discovery tool in clinical steroid investigations even in the era of fast liquid chromatography tandem mass spectrometry (LC/MS/MS)star, open. The Journal of Steroid Biochemistry and Molecular Biology.
URL of Published Version: http://dx.doi.org/10.1016/j.jsbmb.2010.04.010
Identification Number/DOI: 10.1016/j.jsbmb.2010.04.010
Liquid chromatography tandem mass spectrometry (LC/MS/MS) is replacing classical methods for steroid hormone analysis. It requires small sample volumes and has given rise to improved specificity and short analysis times. Its growth has been fueled by criticism of the validity of steroid analysis by older techniques, testosterone measurements being a prime example. While this approach is the gold-standard for measurement of individual steroids, and panels of such compounds, LC/MS/MS is of limited use in defining novel metabolomes. GC/MS, in contrast, is unsuited to rapid high-sensitivity analysis of specific compounds, but remains the most powerful discovery tool for defining steroid disorder metabolomes. Since the 1930s almost all inborn errors in steroidogenesis have been first defined through their urinary steroid excretion. In the last 30 years, this has been exclusively carried out by GC/MS and has defined conditions such as AME syndrome, glucocorticoid remediable aldosteronism (GRA) and Smith–Lemli–Opitz syndrome. Our recent foci have been on P450 oxidoreductase deficiency (ORD) and apparent cortisone reductase deficiency (ACRD).
|Type of Work:||Article|
|Date:||April 2010 (Publication)|
|School/Faculty:||Colleges (2008 onwards) > College of Medical & Dental Sciences|
|Department:||Centre for Endocrinology|
|Subjects:||RC Internal medicine|
|Institution:||University of Birmingham|
|Copyright Holders:||Elsevier Inc|
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