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Complete replication of hepatitis C virus in cell culture.

Lindenbach, Brett D and Evans, Matthew J and Syder, Andrew J and Wölk, Benno and Tellinghuisen, Timothy L and Liu, Christopher C and Maruyama, Toshiaki and Hynes, Richard O and Burton, Dennis R and McKeating, Jane A and Rice, Charles M (2005) Complete replication of hepatitis C virus in cell culture. Science (New York, N.Y.), 309 (5734). pp. 623-6. ISSN 1095-9203

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URL of Published Version: http://www.sciencemag.org/cgi/content/full/309/5734/623?ijkey=JIAV9pnqQNchw&keytype=ref&siteid=sci

Many aspects of the hepatitis C virus (HCV) life cycle have not been reproduced in cell culture, which has slowed research progress on this important human pathogen. Here, we describe a full-length HCV genome that replicates and produces virus particles that are infectious in cell culture (HCVcc). Replication of HCVcc was robust, producing nearly 10(5) infectious units per milliliter within 48 hours. Virus particles were filterable and neutralized with a monoclonal antibody against the viral glycoprotein E2. Viral entry was dependent on cellular expression of a putative HCV receptor, CD81. HCVcc replication was inhibited by interferon-alpha and by several HCV-specific antiviral compounds, suggesting that this in vitro system will aid in the search for improved antivirals.

Type of Work:Article
Date:2005 (Publication)
School/Faculty:Schools (1998 to 2008) > School of Medicine
Department:Immunity and Infection
Subjects:R Medicine (General)
QR355 Virology
QR180 Immunology
QR Microbiology
Institution:University of Birmingham
Copyright Holders:Science Magazine
ID Code:494
Refereed:YES
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