Lindenbach, Brett D and Evans, Matthew J and Syder, Andrew J and Wölk, Benno and Tellinghuisen, Timothy L and Liu, Christopher C and Maruyama, Toshiaki and Hynes, Richard O and Burton, Dennis R and McKeating, Jane A and Rice, Charles M (2005) Complete replication of hepatitis C virus in cell culture. Science (New York, N.Y.), 309 (5734). pp. 623-6. ISSN 1095-9203
| URL of Published Version: http://www.sciencemag.org/cgi/content/full/309/5734/623?ijkey=JIAV9pnqQNchw&keytype=ref&siteid=sci Many aspects of the hepatitis C virus (HCV) life cycle have not been reproduced in cell culture, which has slowed research progress on this important human pathogen. Here, we describe a full-length HCV genome that replicates and produces virus particles that are infectious in cell culture (HCVcc). Replication of HCVcc was robust, producing nearly 10(5) infectious units per milliliter within 48 hours. Virus particles were filterable and neutralized with a monoclonal antibody against the viral glycoprotein E2. Viral entry was dependent on cellular expression of a putative HCV receptor, CD81. HCVcc replication was inhibited by interferon-alpha and by several HCV-specific antiviral compounds, suggesting that this in vitro system will aid in the search for improved antivirals. |
| Type of Work: | Article |
|---|---|
| Date: | 2005 (Publication) |
| School/Faculty: | Schools (1998 to 2008) > School of Medicine |
| Department: | Immunity and Infection |
| Subjects: | R Medicine (General) QR355 Virology QR180 Immunology QR Microbiology |
| Institution: | University of Birmingham |
| Copyright Holders: | Science Magazine |
| ID Code: | 494 |
| Refereed: | YES |
| Local Holdings: |
|
Repository Staff Only: item control page
