ePrints Repository

Probing the mechanisms of electron capture dissociation mass spectrometry with nitrated peptides

Jones, Andrew W. and Cooper, Helen J. (2010) Probing the mechanisms of electron capture dissociation mass spectrometry with nitrated peptides. Physical Chemistry Chemical Physics, 12 (41). p. 13394. ISSN 1463-9076

Loading
PDF (775Kb)

URL of Published Version: http://dx.doi.org/10.1039/C0CP00623H

Identification Number/DOI: 10.1039/C0CP00623H

Previously we have shown that the presence of 3-nitrotyrosine within a peptide sequence severely depletes the peptide backbone fragments typically observed following electron capture dissociation (ECD) mass spectrometry. Instead, ECD of nitrated peptides is characterised by abundant losses of small neutrals (hydroxyl radicals, water and ammonia). Here, we investigate the origin of ammonia loss by comparing the ECD behaviour of lysine- and arginine-containing nitrated peptides, and their N-acetylated counterparts, and nitrated peptides containing no basic amino acid residues. The results reveal that ammonia loss derives from the N-terminus of the peptides, however, the key finding of this work is the insight provided into the hierarchy of various proposed ECD mechanisms: the Utah-Washington mechanism, the electron predator mechanism and the Oslo mechanism.

Type of Work:Article
Date:2010 (Publication)
School/Faculty:Colleges (2008 onwards) > College of Life & Environmental Sciences
Department:School of Biosciences
Subjects:QR Microbiology
Institution:University of Birmingham
Copyright Holders:Royal Society of Chemistry
ID Code:537
Refereed:YES
Local Holdings:
Export Reference As : ASCII + BibTeX + Dublin Core + EndNote + HTML + METS + MODS + OpenURL Object + Reference Manager + Refer + RefWorks
Share this item :
QR Code for this page

Repository Staff Only: item control page