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Identification and structural characterisation of a partially arabinosylated lipoarabinomannan variant isolated from a Corynebacterium glutamicum ubiAmutant

Tatituri, Raju Venkata Veera and Alderwick, Luke J. and Mishra, Arun K. and Nigou, Jerome and Gilleron, Martine and Krumbach, Karin and Hitchen, Paul and Giordano, Assunta and Morris, Howard R. and Dell, Anne and Eggeling, Lothar and Besra, Gurdyal S (2007) Identification and structural characterisation of a partially arabinosylated lipoarabinomannan variant isolated from a Corynebacterium glutamicum ubiAmutant. Microbiology. (Unpublished)

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Identification Number/DOI: 10.1099/mic.0.2007/008078-0

Arabinan polysaccharide side-chains are present in both Mycobacterium tuberculosis and Corynebacterium glutamicum in the heteropolysaccharide arabinogalactan (AG), and in M. tuberculosis in the lipoglycan, lipoarabinomannan (LAM). Herein, we show by quantitative sugar and glycosyl linkage analysis that C. glutamicum possesses a much smaller LAM version, Cg-LAM, characterised by single t-Araf residues linked to the

Type of Work:Article
Date:2007 (Publication)
School/Faculty:Schools (1998 to 2008) > School of Biosciences
Department:School of Biosciences
References:

REFERENCES
Alderwick, L. J., Radmacher, E., Seidel, M. & other authors (2005). Deletion of Cgemb in Corynebacterianeae leads to a novel truncated cell wall arabinogalactan, whereas inactivation of Cg-ubiA results in an arabinan-deficient mutant with a cell wall galactan
core. J Biol Chem 280, 32362-32371.

Alderwick, L. J., Dover, L. G., Seidel, M., Gande, R., Sahm, H., Eggeling, L. & Besra, G. S. (2006a). Arabinan-deficient mutants of Corynebacterium glutamicum and
the consequent flux in decaprenylmonophosphoryl-D-arabinose metabolism. Glycobiology 16, 1073-1081.

Alderwick, L. J., Seidel, M., Sahm, H., Besra, G. S. & Eggeling, L. (2006b). Identification of a novel arabinofuranosyltransferase (AftA) involved in cell wall
arabinan biosynthesis in Mycobacterium tuberculosis. J Biol Chem 281, 15653-15661.

Belanger, A. E., Besra, G. S., Ford, M. E., Mikusova, K., Belisle, J. T., Brennan, P. J. & Inamine, J. M. (1996). The embAB genes of Mycobacterium avium encode an
arabinosyl transferase involved in cell wall arabinan biosynthesis that is the target for the antimycobacterial drug ethambutol. Proc Natl Acad Sci U S A 93, 11919-11924.

Berg, S., Starbuck, J., Torrelles, J. B., Vissa, V. D., Crick, D. C., Chatterjee, D. & Brennan, P. J. (2005). Roles of conserved proline and glycosyltransferase motifs of EmbC in biosynthesis of lipoarabinomannan. J Biol Chem 280, 5651-5663.

Keywords:Corynebacterium glutamicum
Subjects:QR180 Immunology
QR Microbiology
Institution:University of Birmingham
ID Code:9
Refereed:YES
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