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Autoregulation of the Escherichia coli melR promoter: repression involves four molecules of MelR

Samarasinghe, Shivanthi and El-Robh, Mohamed Samir and Grainger, David C. and Zhang, Wenke and Soultanas, Panos and Busby, Stephen J. W. (2008) Autoregulation of the Escherichia coli melR promoter: repression involves four molecules of MelR. Nucleic Acids Research, 36 (8). pp. 2667-2696. ISSN 0305-1048

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URL of Published Version: http://nar.oxfordjournals.org/cgi/content/full/36/8/2667

Identification Number/DOI: 10.1093/nar/gkn119

The Escherichia coli MelR protein is a transcription activator that autoregulates its own promoter by repressing transcription initiation. Optimal repression requires MelR binding to a site that overlaps the melR transcription start point and to upstream sites. In this work, we have investigated the different determinants needed for optimal repression and their spatial requirements. We show that repression requires a complex involving four DNA-bound MelR molecules, and that the global CRP regulator plays little or no role.

Type of Work:Article
Date:16 March 2008 (Publication)
School/Faculty:Schools (1998 to 2008) > School of Biosciences
Department:Molecular Microbiology
Subjects:QR Microbiology
Institution:University of Birmingham
Copyright Holders:Oxford journals
ID Code:91
Refereed:YES
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