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Dissecting the roles of Escherichia coli hydrogenases in biohydrogen production

Redwood, MD and Mikheenko, IP and Sargent, F and Macaskie, LE (2008) Dissecting the roles of Escherichia coli hydrogenases in biohydrogen production. FEMS Microbiology Letters, 278. pp. 48-55.

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URL of Published Version: http://www.ncbi.nlm.nih.gov/pubmed/17995952

Identification Number/DOI: 10.1111/j.1574-6968.2007.00966.x

Escherichia coli can perform at least two modes of anaerobic hydrogen metabolism and expresses at least two types of hydrogenase activity. Respiratory hydrogen oxidation is catalysed by two ‘uptake’ hydrogenase isoenzymes, hydrogenase -1 and -2 (Hyd-1 and -2), and fermentative hydrogen production is catalysed by Hyd-3.
Harnessing and enhancing the metabolic capability of E. coli to perform anaerobic mixed-acid fermentation is therefore an attractive approach for bio-hydrogen
production from sugars. In this work, the effects of genetic modification of the genes encoding the uptake hydrogenases, as well as the importance of preculture
conditions, on hydrogen production and fermentation balance were examined. In suspensions of resting cells pregrown aerobically with formate, deletions in Hyd-3 abolished hydrogen production, whereas the deletion of both uptake hydrogenases improved hydrogen production by 37% over the parent strain. Under fermentative conditions, respiratory H2 uptake activity was absent in strains lacking Hyd-2. The
effect of a deletion in hycA on H2 production was found to be dependent upon environmental conditions, but H2 uptake was not significantly affected by this mutation.

Type of Work:Article
Date:2008 (Publication)
School/Faculty:Colleges (2008 onwards) > College of Life & Environmental Sciences
Department:School of Biosciences
Subjects:Q Science (General)
Institution:University of Birmingham, University of Dundee
Copyright Holders:Elsevier
ID Code:983
Refereed:YES
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