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Polyhydroxybutyrate accumulation by a Serratia sp

Lugg, H and Sammons, RL and Marquis, PM and Hewitt, CJ and Yong, P and Paterson-Beedle, M and Redwood, MD and Stamboulis, A and Kashani, M and Jenkins, M and Macaskie, LE (2008) Polyhydroxybutyrate accumulation by a Serratia sp. Biotechnology Letters, 30 (3). pp. 481-491.

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Identification Number/DOI: DOI 10.1007/s10529-007-9561-9

A strain of Serratia sp. showed intracellular electron-transparent inclusion bodies when incubated in the presence of citrate and glycerol 2-phosphate without nitrogen source following pregrowth under carbon-limitation in continuous culture. About 1.3 mmol citrate were consumed per 450 mg
biomass, giving a calculated yield of maximally 55% of stored material per g of biomass dry wt. The inclusion bodies were stained with Sudan Black and Nile Red (NR), suggesting a lipid material, which was confirmed as polyhydroxybutyrate (PHB) by analysis of molecular fragments by GC and by FTIR spectroscopy of isolated bio-PHB in comparison with reference material. Multi-parameter flow cytometry in conjunction with NR fluorescence, and electron microscopy, showed that not all cells contained heavy PHB bodies, suggesting the potential for increasing
the overall yield. The economic attractiveness is
enhanced by the co-production of nanoscale hydroxyapatite
(HA), a possible high-value precursor for bone replacement materials.

Type of Work:Article
Date:2008 (Publication)
School/Faculty:Colleges (2008 onwards) > College of Life & Environmental Sciences
Department:School of Biosciences
Subjects:Q Science (General)
Institution:University of Birmingham
Copyright Holders:Kluwer
ID Code:995

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  • Polyhydroxybutyrate accumulation by a Serratia sp. (deposited 18 Jan 2012 13:55) [Currently Displayed]
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